Synaptic Systems

Cat. No.: 322 102

Amount: 200 µl

Price: $360.00

Cat. No. 322 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: external data (see remarks) IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 903 to 960 from mouse GabaBR1 (UniProt Id: Q9WV18)
Reactivity	Reacts with: rat, mouse (Q9WV18). Other species not tested yet.
Specificity	Detects both isoforms GABABR 1a and 1b.
Remarks	IHC: This antibody has been successfully applied and published for this method by customers (see application-specific references). It has not been validated using our standard protocols.
Data sheet	322_102.pdf

References for GABABR1 - 322 102

Inhibitory control in neuronal networks relies on the extracellular matrix integrity.
Dzyubenko E, Fleischer M, Manrique-Castano D, Borbor M, Kleinschnitz C, Faissner A, Hermann DM
Cellular and molecular life sciences : CMLS (2021) 7814: 5647-5663. 322 102 WB, IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 322 102 IHC; tested species: mouse

Cat. No.: 322 102

Amount: 200 µl

Price: $360.00

ICC

Background

Metabotropic g-aminobutyric acid type B receptors (GABABRs) are involved in the modulation of synaptic transmission and activity of cerebellar and thalamic neurons.
In contrast to the ionotrophic GABA-A receptors they are linked to G-proteins underlying the slow inhibitory postsynaptic potentials.
GABABR 1 and GABABR 2 have been shown to form heterodimers.

1 ) Kulik A et al., Eur. J. Neurosci. (2002)
2 ) Filippov AK et al., J. Neurosci. (2000)

Protocols

ICC

GABABR1 antibody - 322 102

References for GABABR1 - 322 102