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MPP5 (PALS1) antibody - 220 003

MPP5 is a multi adaptor protein
Rabbit polyclonal purified antibody
Cat. No.: 220 003
Amount: 50 µg
Price: $380.00
Cat. No. 220 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 2 to 244 from human MPP5 (UniProt Id: Q8N3R9)
Reactivity Reacts with: human (Q8N3R9), rat, mouse (Q9JLB2), pig, cow.
Other species not tested yet.
Remarks

IHC: For optimal results in retina tissue, follow the retina protocol according to Gierke et al. 2023.
Data sheet 220_003.pdf
Cat. No.: 220 003
Amount: 50 µg
Price: $380.00
WB
ICC
ICC
IHC
Background

The membrane palmitoylated protein 5 (MPP 5), also referred to as PALS 1, belongs to the membrane associated guanylate kinase (MAGUK) protein family. It is a multi adaptor protein that interacts via its PSD-95/Dlg/ZO-1 (PDZ) and L-27 domains with the proteins Crb 1, Crb 3 and Patj. It has been shown to be involved in the establishment of apicobasal cell polarity.