Synaptic Systems - Calretinin

Cat. No.: 214 106

Amount: 200 µl

Price: $365.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 214 106	200 µl antibody, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 gallery iDISCO: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. iDISCO is a fast and cost-effective sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. The clearing is carried out by benzyl-ether treatment. For additional experimental details, please refer to the „Remarks“ section or visit https://idisco.info.
Immunogen	Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity	Reacts with: rat (P47728), mouse (Q08331). Other species not tested yet.
Matching control protein/peptide	214-1P
Remarks	iDISCO: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	214_106.pdf

References for Calretinin - 214 106

Brevican, Neurocan, Tenascin-C, and Tenascin-R Act as Important Regulators of the Interplay Between Perineuronal Nets, Synaptic Integrity, Inhibitory Interneurons, and Otx2.
Mueller-Buehl C, Reinhard J, Roll L, Bader V, Winklhofer KF, Faissner A
Frontiers in cell and developmental biology (2022) 10: 886527. 214 106 WB, IHC; tested species: mouse

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 214 106 ICC; tested species: rat

Neuroinflammation causes mitral cell dysfunction and olfactory impairment in a multiple sclerosis model.
Schubert C, Schulz K, Sonner JK, Hadjilaou A, Seemann AL, Gierke J, Vieira V, Meurs N, Woo MS, Lohr C, Morellini F, et al.
Journal of neuroinflammation (2025) 221: 71. 214 106 IHC; tested species: mouse

Gating of hair cell Ca2+ channels governs the activity of cochlear neurons.
Karagulyan N, Thirumalai A, Michanski S, Qi Y, Fang Q, Wang H, Ortner NJ, Striessnig J, Strenzke N, Wichmann C, Hua Y, et al.
Science advances (2025) 1125: eadu7898. 214 106 IHC; tested species: mouse

Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 106 IHC; tested species: mouse

Overloaded Adeno-Associated Virus as a Novel Gene Therapeutic Tool for Otoferlin-Related Deafness.
Rankovic V, Vogl C, Dörje NM, Bahader I, Duque-Afonso CJ, Thirumalai A, Weber T, Kusch K, Strenzke N, Moser T
Frontiers in molecular neuroscience (2020) 13: 600051. 214 106 IHC, iDISCO; tested species: mouse

Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 214 106 IHC; tested species: mouse

Cat. No.: 214 106

Amount: 200 µl

Price: $365.00

Ventral hippocampal projections to the medial prefrontal cortex regulate social memory.
Phillips ML, Robinson HA, Pozzo-Miller L
eLife (2019) 8: . 214 106 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.

1 ) Klejbor I et al., J. Physiol. Pharmacol. (2006)
2 ) Haworth R et al., Exp. Toxicol. Pathol. (2006)
3 ) Schmidt H et al., J. Physiol. (Lond.) (2003)
4 ) Barski JJ et al., J. Neurosci. (2003)
5 ) Schwaller B et al., Cerebellum (2002)
6 ) Faucheux C et al., Biochem. J. (1998)
7 ) Lee YS et al., Proc. Natl. Acad. Sci. U.S.A. (1987)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Calretinin antibody - 214 106

References for Calretinin - 214 106