CaMK1 antibody - 211 005

CaMK1 is a calmodulin-dependent kinase

Guinea pig polyclonal purified antibody

Cat. No.: 211 005

Amount: 50 µg

Price: $465.00

Cat. No. 211 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: external data (see remarks) IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 374 from mouse CaMK1 (UniProt Id: Q91YS8)
Reactivity	Reacts with: human (Q14012), rat (Q63450), mouse (Q91YS8). Other species not tested yet.
Specificity	Specifiy for CaMK 1, recognizes all isoforms (α, β, γ, and δ).
Remarks	IHC: This antibody has been successfully applied and published for this method by customers (see application-specific references). It has not been validated using our standard protocols.
Data sheet	211_005.pdf

References for CaMK1 - 211 005

Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area.
Wang TA, Teo CF, Åkerblom M, Chen C, Tynan-La Fontaine M, Greiner VJ, Diaz A, McManus MT, Jan YN, Jan LY
Neuron (2019) : . 211 005 IHC; tested species: mouse

Cat. No.: 211 005

Amount: 50 µg

Price: $465.00

ICC

Background

Calcium- and calmodulin-dependent kinase 1 (CaMK 1) is a serine/threonine kinase that belongs to the family of calcium-dependent kinases consisting of CaMK 2, CaMK 4, and CaMKK (CaMK-kinase). Four different isoforms (CaMK 1 α, β, γ, and δ) derived from independent genes have been described. CaMK 1 is the most abundant isoform in the central nervous system and is involved in cellular differentiation processes.

1 ) Uboha NV et al., J. Neurosci. (2007)
2 ) Tokumitsu H et al., J. Biol. Chem. (2005)
3 ) Condon JC et al., Endocrinology (2002)
4 ) Matsushita M et al., J. Biol. Chem. (1999)
5 ) Matsushita M et al., J. Biol. Chem. (1998)
6 ) Picciotto MR et al., J. Biol. Chem. (1993)

Protocols

ICC

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