Tailor-made Antibodies
and Tools for Life Science
Home | | | | | Technical Support

mGluR6 antibody - 191 603

mGluRs are metabotropic glutamate receptors involved in excitatory neurotransmission
Rabbit polyclonal purified antibody
Cat. No.: 191 603
Amount: 50 µg
Price: $380.00
Cat. No. 191 603 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 5000 gallery  
IHC-P: not recommended
IHC-Fr: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse mGluR6 (UniProt Id: Q5NCH9)
Reactivity Reacts with: mouse (Q5NCH9), rat (P35349).
Other species not tested yet.
Remarks

IHC: For optimal results in retina tissue, follow the retina protocol according to Gierke et al. 2023.
IHC-Fr: Acetone fixation is recommended.
The following fixatives are not advised: methanol-acetone, 4% formaldehyde/PFA, methanol

Data sheet 191_603.pdf
Cat. No.: 191 603
Amount: 50 µg
Price: $380.00