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VGLUT1 (SLC17A7) antibody - 135 316 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Chicken polyclonal IgY fraction
Cat. No.: 135 316
Amount: 200 µl
Price: $370.00
Cat. No. 135 316 200 µl antibody, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
IHC-Fr: 1 : 500 up to 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-0P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB: To avoid protein aggregation, do not heat samples for SDS-PAGE.
IHC-Fr: 4% formaldehyde/PFA fixation is recommended.
Data sheet 135_316.pdf

References for VGLUT1 - 135 316

Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons.
Tereshko L, Gao Y, Cary BA, Turrigiano GG, Sengupta P
eLife (2021) 10: . 135 316 ICC; tested species: rat
Isolation of Human CD49f+ Astrocytes and In Vitro iPSC-Based Neurotoxicity Assays.
Barbar L, Rusielewicz T, Zimmer M, Kalpana K, Fossati V
STAR protocols (2020) 13: 100172. 135 316 ICC; tested species: human
Real-time mechanisms of exacerbated synaptic remodeling by microglia in acute models of systemic inflammation and tauopathy.
Cangalaya C, Wegmann S, Sun W, Diez L, Gottfried A, Richter K, Stoyanov S, Pakan J, Fischer KD, Dityatev A
Brain, behavior, and immunity (2023) : . 135 316 IHC; tested species: mouse
iPSC-derived microglia carrying the TREM2 R47H/+ mutation are proinflammatory and promote synapse loss.
Penney J, Ralvenius WT, Loon A, Cerit O, Dileep V, Milo B, Pao PC, Woolf H, Tsai LH
Glia (2023) : . 135 316 IHC; tested species: mouse
Electrical stimulation affects the differentiation of transplanted regionally specific human spinal neural progenitor cells (sNPCs) after chronic spinal cord injury.
Patil N, Korenfeld O, Scalf RN, Lavoie N, Huntemer-Silveira A, Han G, Swenson R, Parr AM
Stem cell research & therapy (2023) 141: 378. 135 316 IHC; tested species: rat
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 135 316 IHC; tested species: mouse
AAV9-Mediated Delivery of miR-23a Reduces Disease Severity in Smn2B-/SMA Model Mice.
Kaifer KA, Villalón E, O'Brien BS, Sison SL, Smith CE, Simon ME, Marquez J, O'Day S, Hopkins AE, Neff R, Rindt H, et al.
Human molecular genetics (2019) : . 135 316 IHC; tested species: mouse
Transfer of the Experimental Autoimmune Glaucoma Model from Rats to Mice-New Options to Study Glaucoma Disease.
Reinehr S, Reinhard J, Wiemann S, Hesse K, Voss C, Gandej M, Dick HB, Faissner A, Joachim SC
International journal of molecular sciences (2019) 2010: . 135 316 IHC; tested species: mouse
Presynaptic PRRT2 deficiency causes cerebellar dysfunction and paroxysmal kinesigenic dyskinesia.
Calame DJ, Xiao J, Khan MM, Hollinsworth TJ, Xue Y, Person AL, LeDoux MS
Neuroscience (2020) : . 135 316 IHC-P; tested species: mouse
Cat. No.: 135 316
Amount: 200 µl
Price: $370.00
Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons.
Tereshko L, Gao Y, Cary BA, Turrigiano GG, Sengupta P
eLife (2021) 10: . 135 316 ICC; tested species: rat
Isolation of Human CD49f+ Astrocytes and In Vitro iPSC-Based Neurotoxicity Assays.
Barbar L, Rusielewicz T, Zimmer M, Kalpana K, Fossati V
STAR protocols (2020) 13: 100172. 135 316 ICC; tested species: human
Real-time mechanisms of exacerbated synaptic remodeling by microglia in acute models of systemic inflammation and tauopathy.
Cangalaya C, Wegmann S, Sun W, Diez L, Gottfried A, Richter K, Stoyanov S, Pakan J, Fischer KD, Dityatev A
Brain, behavior, and immunity (2023) : . 135 316 IHC; tested species: mouse
iPSC-derived microglia carrying the TREM2 R47H/+ mutation are proinflammatory and promote synapse loss.
Penney J, Ralvenius WT, Loon A, Cerit O, Dileep V, Milo B, Pao PC, Woolf H, Tsai LH
Glia (2023) : . 135 316 IHC; tested species: mouse
Electrical stimulation affects the differentiation of transplanted regionally specific human spinal neural progenitor cells (sNPCs) after chronic spinal cord injury.
Patil N, Korenfeld O, Scalf RN, Lavoie N, Huntemer-Silveira A, Han G, Swenson R, Parr AM
Stem cell research & therapy (2023) 141: 378. 135 316 IHC; tested species: rat
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 135 316 IHC; tested species: mouse
AAV9-Mediated Delivery of miR-23a Reduces Disease Severity in Smn2B-/SMA Model Mice.
Kaifer KA, Villalón E, O'Brien BS, Sison SL, Smith CE, Simon ME, Marquez J, O'Day S, Hopkins AE, Neff R, Rindt H, et al.
Human molecular genetics (2019) : . 135 316 IHC; tested species: mouse
Transfer of the Experimental Autoimmune Glaucoma Model from Rats to Mice-New Options to Study Glaucoma Disease.
Reinehr S, Reinhard J, Wiemann S, Hesse K, Voss C, Gandej M, Dick HB, Faissner A, Joachim SC
International journal of molecular sciences (2019) 2010: . 135 316 IHC; tested species: mouse
Presynaptic PRRT2 deficiency causes cerebellar dysfunction and paroxysmal kinesigenic dyskinesia.
Calame DJ, Xiao J, Khan MM, Hollinsworth TJ, Xue Y, Person AL, LeDoux MS
Neuroscience (2020) : . 135 316 IHC-P; tested species: mouse
WB
ICC
IHC
IHC-P
IHC-Fr
Background

The vesicular glutamate transporter 1 VGLUT1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT2, VGLUT1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT1 and VGLUT2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.
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