Synaptic Systems - GABA-A receptor alpha1

Cat. No.: 224 211

Amount: 100 µg

Price: $420.00

Cat. No. 224 211	100 µg purified IgM, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 gallery IHC-P: not tested yet EM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone	53D3
Subtype	IgM (κ light chain)
Immunogen	Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor α1. (UniProt Id: P62813)
Reactivity	Reacts with: rat (P62813), mouse (P62812). Other species not tested yet.
Matching control protein/peptide	224-2P
Remarks	ICC: This antibody can be used for the surface staining of living cells. EM: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	224_211.pdf

References for GABA-A receptor alpha1 - 224 211

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 224 211 WB

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism.
Li J, Xie Y, Cornelius S, Jiang X, Sando R, Kordon SP, Pan M, Leon K, Südhof TC, Zhao M, Araç D, et al.
Nature communications (2020) 111: 2140. 224 211 ICC; tested species: mouse

Brefeldin A-inhibited guanine nucleotide exchange protein 3 is localized in lysosomes and regulates GABA signaling in hippocampal neurons.
Liu T, Li H, Hong W, Han W
Journal of neurochemistry (2016) 1395: 748-756. 224 211 ICC

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 211 EM

Cat. No.: 224 211

Amount: 100 µg

Price: $420.00

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 224 211 WB

CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
Cook SG, Buonarati OR, Coultrap SJ, Bayer KU
Science advances (2021) 716: . 224 211 WB, ICC; tested species: mouse

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 224 211 EM

ICC

IHC

Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Laurie DJ et al., J. Neurosci. (1992)
2 ) Laurie DJ et al., J. Neurosci. (1992)
3 ) Wisden W et al., J. Neurosci. (1992)
4 ) Lüddens H et al., Nature (1990)
5 ) Sassoè-Pognetto M et al., Ann. N. Y. Acad. Sci. (2009)
6 ) Viltono L et al., J. Comp. Neurol. (2008)
7 ) Kralic JE et al., J. Comp. Neurol. (2006)
8 ) Essrich C et al., Nat. Neurosci. (1998)
9 ) Fritschy JM et al., J. Comp. Neurol. (1995)
10 ) Benke D et al., J. Biol. Chem. (1994)

Protocols

ICC

IHC

OTHER

Preabsorbtion

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GABA-A receptor alpha1 antibody extracellular - 224 211

References for GABA-A receptor alpha1 - 224 211