Synaptic Systems - Calretinin

Cat. No.: 214 111

Amount: 100 µg

Price: $420.00

Cat. No. 214 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 1000 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	37C9
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant mouse Calretinin (UniProt Id: Q08331)
Reactivity	Reacts with: rat (P47728), mouse (Q08331), human (P22676), zebrafish. Other species not tested yet.
Specificity	Specific for calretinin / calbindin D29k.
Matching control protein/peptide	214-1P
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is not advised.
Data sheet	214_111.pdf

References for Calretinin - 214 111

Non-canonical function of ADAM10 in presynaptic plasticity.
Bär J, Fanutza T, Reimann CC, Seipold L, Grohe M, Bolter JR, Delfs F, Bucher M, Gee CE, Schweizer M, Saftig P, et al.
Cellular and molecular life sciences : CMLS (2024) 811: 342. 214 111 ICC; tested species: mouse

Role of PKN1 in Retinal Cell Type Formation.
Brunner M, Lang L, Künkel L, Weber D, Safari MS, Baier-Bitterlich G, Zur Nedden S
International journal of molecular sciences (2024) 255: . 214 111 IHC; tested species: mouse

Neuronal integration in the adult mouse olfactory bulb is a non-selective addition process.
Platel JC, Angelova A, Bugeon S, Wallace J, Ganay T, Chudotvorova I, Deloulme JC, Béclin C, Tiveron MC, Coré N, Murthy VN, et al.
eLife (2019) 8: . 214 111 IHC; tested species: mouse

Delivery of different genes into pre- and post-synaptic neocortical interneurons connected by GABAergic synapses.
Nagayach A, Singh A, De Blas AL, Geller AI
PloS one (2019) 145: e0217094. 214 111 IHC; tested species: rat

Characterization of perinatally born glutamatergic neurons of the mouse olfactory bulb based on NeuroD6 expression reveals their resistance to sensory deprivation.
Angelova A, Platel JC, Béclin C, Cremer H, Coré N
The Journal of comparative neurology (2019) 5277: 1245-1260. 214 111 IHC; tested species: mouse

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 214 111 IHC; tested species: mouse

NCAM Regulates Inhibition and Excitability in Layer 2/3 Pyramidal Cells of Anterior Cingulate Cortex.
Zhang X, Sullivan CS, Kratz MB, Kasten MR, Maness PF, Manis PB
Frontiers in neural circuits (2017) 11: 19. 214 111 IHC; tested species: mouse

Cat. No.: 214 111

Amount: 100 µg

Price: $420.00

ICC

IHC

IHC-P

Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: Calretinin, also referred to as calbindin D29k, calbindin 2, CALB 2, CAL 2, and CAB 29, and calbindin D28k. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Klejbor I et al., J. Physiol. Pharmacol. (2006)
2 ) Haworth R et al., Exp. Toxicol. Pathol. (2006)
3 ) Schmidt H et al., J. Physiol. (Lond.) (2003)
4 ) Barski JJ et al., J. Neurosci. (2003)
5 ) Schwaller B et al., Cerebellum (2002)
6 ) Faucheux C et al., Biochem. J. (1998)
7 ) Lee YS et al., Proc. Natl. Acad. Sci. U.S.A. (1987)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Calretinin antibody - 214 111

References for Calretinin - 214 111